Shared by Downes Lab (Saint Louis University)

Plasmid Name pACT2.2gtwy+UBC9* S52R
Alt Name/ID p118
Backbone pACT2.2gtwy
Backbone Size 8187
Gene/Insert UBC9* S52R
Alt Insert Name At4g27960
Insert Size 537
Species A. thaliana (mustard weed)
GenBank IDs DQ027023
Entrez Gene ID UBC9 (Arabidopsis thaliana) (AT4G27960, T13J8.70, T13J8_70, UBIQUITIN CONJUGATING ENZYME 9, UBIQUITIN-PROTEIN LIGASE)
Mutations/Deletions S52R
Tag 1 (e.g. GFP, HA) GAL4 AD
Location of Tag 1 N terminal on backbone
5' Sequence Primer CTCTATGGCTTACCCATACGATGTTCC
Bacterial Resistance Ampicillin
Selectable Marker LEU2
Plasmid Sequence View Sequence
Source Created in Lab
Source, Specify R. Tague 09.09.07
Notes Product of Site-directed Mutagenesis (Stratagene) off pACT2.2gtwy+UBC9* (p88) using primers 210 and 211. Sequenced Correct 09.10.07
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