An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins.

He X, Wang J, Steele J, Sun X, Nie W, Tzipori S, Feng H
Associated Labs:
Feng Lab (University of Maryland, Baltimore)
J Microbiol Methods. 2009 Jul . 78(1):97-100.
PMID: 19393695
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.

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