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|Description||Mouse Monoclonal anti-TREM-1 (TREM-26), conjugated to Biotin|
|Immunogen||Recombinant human TREM-1|
|Applications||Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and/or ELISA analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. For ELISA detection applications, the suggested use of this reagent is 0.5-2 microgram/ml. To obtain a linear standard curve, serial dilutions of TREM-1 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.|
flow cytometry / FACS analysis
|Storage||Store at 4C. Do not freeze. Do not freeze.|
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.|
|Packaging||0.1 mg Immunogen affinity purified Mouse antisera|
|Control||Highly expressed in adult liver, lung, and spleen, lower expression in lymph node, placenta, spinal|
|Reference(s)||1. Bouchon, A., et al., 2001. Nature 410:1103.2. Bouchon, A., et al., 2000. J. Immunol. 164:4991.3. Gingras, M.-C., et al., 2002. Mol. Immunol. 38:817.|
|Background||TREM-1 is a 30 kD glycoprotein also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. It is a Type I membrane protein that contains an immunoglobulin-like V-type domain. Alternatively spliced protein variant may be secreted. TREM-1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM-1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM-1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM-1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock. This antibody has been shown to be useful for flow cytometry and activation of monocytes and granulocytes.|
|Alternative Names||anti-Triggering receptor expressed on myeloid cells 1 antibody, anti-triggering receptor expressed on monocytes 1 antibody, anti-TREM 1 antibody|
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