|Learn about LabLife||Community||Product Search||
|Gene/Insert||cMyc, KLF4, Oct4, Sox2|
|Species||M. musculus (mouse)|
|Tag 1 (e.g. GFP, HA)||IRES-bGeo|
|Location of Tag 1||C terminal on backbone|
|Cloning Site 5'||attB1|
|5' Site Destroyed||No|
|Cloning Site 3'||attB2|
|3' Site Destroyed||No|
|5' Sequence Primer||M13 F, T7|
|3' Sequence Primer||M13 R, T3|
|Special Growth Conditions||Plasmid grows in standard E. coli at 37C.|
|Notes||Type of vector: Mammalian expression|
This plasmid was derived from a plasmid from MKOS liked by 2A peptides obtained from Keisuke Kaji et al (Nature. 2009 Mar 1) piggyBac terminal repeats obtained from Pentao Liu at Sanger.
Additional reference, Kaji et al Nature. 2009 Mar 1.\n\n\nAny PB vector has to have the PBase vector to 'piggyBac transpose' the\nconstruct into the genome. You can get this vector, named pCyL43, from the Sanger institute's plasmid repository at http://www.sanger.ac.uk/technology/clonerequests/\n\nThere is an Sanger MTA that must be signed and is available on their website.
|Plasmid Sequence||View Sequence|
Product data compiled on LabLife is for informational purposes. Please visit the vendor's website for more details and ordering.
Share your experiences with other scientists! Please log in to submit a review.
No reviews yet.